HOW TO USE:

(A) the cropped images are uploaded and then the auto-select mode is applied. The color value is extracted from the reaction chamber and the hue value is computed in the mobile qLAMP app. (B) With the mode of ‘Standard Calibration Curve’, the serially diluted samples generated respective qLAMP profiles and the threshold value which is determined by the second derivative maximum revealed the threshold time for each diluted sample. Thus, the standard curve plotting the threshold time versus the logarithm of DNA copy number can be generated, the black line represents the linear fit to the data, the red dotted lines indicate the 95% confidence interval. (C) With the “Quantification of Real Sample” mode, the qLAMP profile of the real sample is produced, and the threshold time is decided by the threshold value. The threshold time is marked in the calibration curve, showing the DNA copy number in the real sample.

The computer code and source code of the app is available at https://github.com/nqh2412/qLAMP/