Integrative NanoBioMEMS - Voice Control

Function of the speech recognition of the smartphone to automatically operate a portable sample pretreatment microfluidic system

Khang Hoang Bui & Tae Seok Seo

const int valve1 = 12;

const int valve2 = 11;

const int valve3 = 10;

const int valve4 = 9;

const int valve5 = 8;

const int valve6 = 7;

const int valve7 = 6;

const int valve8 = 5;

// prepare, all block

void setup() {

Serial.begin(9600);

pinMode(valve1, OUTPUT);

pinMode(valve2, OUTPUT);

pinMode(valve3, OUTPUT);

pinMode(valve4, OUTPUT);

pinMode(valve5, OUTPUT);

pinMode(valve6, OUTPUT);

pinMode(valve7, OUTPUT);

pinMode(valve8, OUTPUT);

digitalWrite(valve1, LOW);

digitalWrite(valve2, LOW);

digitalWrite(valve3, LOW);

digitalWrite(valve4, LOW);

digitalWrite(valve5, LOW);

digitalWrite(valve6, LOW);

digitalWrite(valve7, LOW);

digitalWrite(valve8, LOW);

}

void loop() {

char c = Serial.read();

//FastestTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

if(c == 'a'){

digitalWrite(valve7, HIGH);

// sample solution - solution 1 loading

digitalWrite(valve2, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(6000);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve2, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//washing 1

delay(1000);

digitalWrite(valve4, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(6000);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve4, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Washing 2

delay(1000);

digitalWrite(valve3, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(6000);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve3, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Elution

digitalWrite(valve1, HIGH);

digitalWrite(valve5, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(6000);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve1, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve5, LOW);

digitalWrite(valve7, LOW);

}

//Normallllllllllllllllllllllllllllllllllllllllllllll

else if(c == 'b'){

digitalWrite(valve7, HIGH);

digitalWrite(valve2, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve2, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//washing 1

delay(1000);

digitalWrite(valve4, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve4, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Washing 2

delay(1000);

digitalWrite(valve3, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve3, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Elution

digitalWrite(valve1, HIGH);

digitalWrite(valve5, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(1000);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve1, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve5, LOW);

digitalWrite(valve7, LOW);

}

//SLOWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWW

else if(c == 'c'){

digitalWrite(valve7, HIGH);

digitalWrite(valve2, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve2, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//washing 1

delay(1000);

digitalWrite(valve4, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve4, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Washing 2

delay(1000);

digitalWrite(valve3, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve3, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Elution

digitalWrite(valve1, HIGH);

digitalWrite(valve5, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(500);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve1, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve5, LOW);

digitalWrite(valve7, LOW);

}

//SLOWEST

if(c == 'd'){

digitalWrite(valve7, HIGH);

// sample solution - solution 1 loading

digitalWrite(valve2, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve2, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//washing 1

delay(1000);

digitalWrite(valve4, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve4, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Washing 2

delay(1000);

digitalWrite(valve3, HIGH);

digitalWrite(valve6, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve3, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve6, LOW);

//Elution

digitalWrite(valve1, HIGH);

digitalWrite(valve5, HIGH);

delay(1000);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

digitalWrite(valve8, HIGH);

delay(250);

digitalWrite(valve8, LOW);

delay(500);

///clean

digitalWrite(valve8, HIGH);

delay(3000);

digitalWrite(valve1, LOW);

digitalWrite(valve8, LOW);

digitalWrite(valve5, LOW);

digitalWrite(valve7, LOW);

}

else{

}

delay(25);

}

Function of the speech recognition of the smartphone to automatically operate a portable sample pretreatment microfluidic system

Khang Hoang Bui & Tae Seok Seo

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